output.Rmd
The CAMDAC pipeline returns a structured directory at the outdir
from the CamConfig()
object. The pipeline returns files unique to the RRBS and WGBS modules with the general structure:
└── <CamSample.patient_id>
├── Allelecounts
│ ├── <CamSample.id>
├── Copynumber
│ ├── <CamSample.id>
└── Methylation
└── <CamSample.id>
The sections below describe each results file in more detail. Next, see vignette("questions")
for frequently asked questions or vignette("experimental")
for details on experimental CAMDAC features.
results/
└── P
├── Allelecounts
│ ├── N
│ │ └── P.N.SNPs.CpGs.all.sorted.RData
│ └── T
│ └── P.T.SNPs.CpGs.all.sorted.RData
├── Copy_number
│ ├── N
│ │ ├── fragment_length_histogram.pdf
│ │ ├── msp1_fragments_RRBS.RData
│ │ ├── P_N_normal_SNP_data.pdf
│ │ ├── P.N.SNPs.RData
│ │ └── Rplots.pdf
│ └── T
│ ├── fragment_length_histogram.pdf
│ ├── msp1_fragments_RRBS.RData
│ ├── P_T_SNP_data.pdf
│ ├── P.T.ACF.and.ploidy.txt
│ ├── P.T.ascat.bc.RData
│ ├── P.T.ascat.frag.RData
│ ├── P.T.ascat.output.RData
│ ├── P.T.ASCATprofile.png
│ ├── P.T.ASPCF.png
│ ├── P.T.BAF.PCFed.txt
│ ├── P.T.germline.png
│ ├── P.T.LogR.PCFed.txt
│ ├── P.T.rawprofile.png
│ ├── P.T.SNPs.RData
│ ├── P.T.sunrise.png
│ ├── P.T.tumour.png
│ └── Rplots.pdf
└── Methylation
├── N
│ ├── dt_normal_m.RData
│ └── P_N_methylation_rate_summary.pdf
└── T
├── CAMDAC_DMPs.bed
├── CAMDAC_purified_tumour.bed
├── CAMDAC_results_per_CpG.RData
├── P_T_DMP_stats.txt
├── P_T_methylation_rate_summary.pdf
├── purified_tumour.RData
└── tumour_versus_normal_methylomes.pdf
File | Description |
---|---|
P.T.SNPs.CpGs.all.sorted.RData |
Allele counts for a sample. Generated by processing BAM file |
P.T.ascat.output.RData |
ASCAT copy number results |
P.T.ASCATprofile.png |
ASCAT copy number profile |
dt_normal_m.RData |
Bulk normal DNA methylation data |
purified_tumour.RData |
CAMDAC-purified DNA methylation rates |
CAMDAC_results_per_CpG.fst |
CAMDAC deconvolution and differential methylation results |
CAMDAC outputs are written in the directory given by config$outdir
in the format PATIENT/DATASET/SAMPLE/
:
└── P
├── Allelecounts
│ ├── N
│ │ └── P.N.SNPs.CpGs.all.sorted.csv.gz
│ └── T
│ └── P.T.SNPs.CpGs.all.sorted.csv.gz
├── Copynumber
│ ├── N
│ │ └── P.N.SNPs.csv.gz
│ └── T
│ ├── ascat
│ ├── battenberg
│ ├── P.T.cna.txt
│ ├── P.T.SNPs.csv.gz
│ └── P.T.tnSNP.csv.gz
└── Methylation
├── N
│ └── P.N.m.csv.gz
└── T
├── P.T.CAMDAC_annotated_DMRs.fst
├── P.T.CAMDAC_results_per_CpG.fst
├── P.T.m.csv.gz
└── P.T.pure.csv.gz
File | Description |
---|---|
P.T.SNPs.CpGs.all.sorted.csv.gz |
Allele counts for a sample. Generated by processing BAM file |
P.T.SNPs.csv.gz |
SNP counts for a sample. |
P.T.cna.txt |
CAMDAC CNA result |
P.T.m.csv.gz |
Bulk methylation data |
P.T.m.pure.csv.gz |
CAMDAC-deconvolved methylation data |
P1.T.CAMDAC_results_per_CpG.fst |
CAMDAC differentially methylated cytosines |
P1.T.CAMDAC_annotated_DMRs.fst |
CAMDAC differentially methylated regions |
It is possible to manually override outputs for runs. See vignette("questions")
for more details.